Journal: Pathogens

Article Title: Genetic Underpinnings of Carotenogenesis and Light-Induced Transcriptome Remodeling in the Opportunistic Pathogen Mycobacterium kansasii

doi: 10.3390/pathogens12010086

Figure Lengend Snippet: Proposed carotenoid biosynthesis pathway of M. kansasii . ( a ) The scheme highlights the functions of the M. kansasii enzymes encoded by the genes described in the presented work, in the context of the general bacterial carotenogenesis pathway . Synthesis of geranylgeranyl diphosphate might take place via the predicted geranylgeranyl diphosphate synthase CrtE or by alternative CrtE-independent routes with putative trans -isoprenyl diphosphate synthases we identified by sequence analysis. The final pathway product shown is β-carotene, the major carotenoid produced by M. kansasii . ( b ) Pairwise amino acid sequence identity matrix for predicted trans -isoprenyl diphosphate synthases of M. kansasii ( Mk , names in red) and M. tuberculosis ( Mt , names in blue). Proteins are labeled with their locus tags and assigned or herein proposed protein names for Mt and Mk , respectively. Amino acid identity percentages of the best Mk - Mt pairs are highlighted (red boxes). Sequence alignment was done with the Clustal W algorithm embedded in the MegAlign application of the DNASTAR Lasergene software.

Article Snippet: DNA and protein sequence alignments were performed using BLAST or the DNASTAR Lasergene software package (DNASTAR Inc., Madison, WI, United States).

Techniques: Sequencing, Produced, Labeling, Software